Amar Mansour Hamrouni, Ph.D
Al Ain Campus
Ph.D. Pharmacology, Cardiff University, Wales, UK
MPhil. Pharmacology, Cardiff University, Wales, UK
B.Pharm. University of Tripoli - Libya
Functional pharmacological experiments characterizing muscarinic receptors using different animal models. Pharmacological evaluation of M3 antagonists used for the treatment of overactive bladder, M1 agonists used for the treatment of Alzheimer’s disease and agents of potential use as anti-inflammatory and antiulcer.
- Sara Al Rawashdah, Amar Hamrouni, Bassem Sadek,Mohammad A. Ghattas, Rawad Amer (2019) Molecular modelling studies on ɑ7 nicotinic receptor allosteric modulators yields novel filter-based virtual screening protocol. Journal of molecular Graphics and Modelling. volume 92, 44-55.
- AR, A., Amar .M, Hamrouni, MM, A., & AM, T. (2019) Osteoporosis Related Knowledge and Attitudes: The Scenario in Tripoli, Libya. Journal of Medical Biomedical and Applied Sciences. 7(8), 272-280.
- Abduelmula Abduelkarem1, Amar Hamrouni (2016) The choice of pharmacy profession as a career: UAE experience. Asian Journal of Pharmaceutical and Clinical Research, Vol 9, Issue 4. 1-7.
- Khaled A. Shakshak, Ali M. Afan, Abdurazag A. Auzi, and Amar hamrouni (2014) The hypoglycemic effect of Libyan Truffle (Terfezia Boudieri) in experimentally induced diabetic rats. Tripolitana Medical Journal, Vol. 3, No. 1, pp. 1-4.
- Bassem Sadek, Amar Mansour Hamrouni, Abdu Adem, (2013) Anti-inflammatory agents of the carbamoylmethyl ester class: synthesis, characterization, and pharmacological evaluation. Journal of Inflammation Research, 6, 35-43.
- F. Musa, A. Alatery, S. Aburawi, A. Alzatreny, A. Hamrouni and A. Auzi (2012) Phytochemical, Antioxidant, Antibacterial and Anti-Inflammatory Investigation of the Methanolic Extract of Amaranthus Tricolor Seed. Tripolitana Medical Journal, Vol. 1, No. 2, pp. 94-99
- Abdulelkarem, A., Hamrouni, A. M., et al. (2010) Prescribing Pattern of Selected Agents at General Medicine Department, Tripoli Central Hospital, Libya.International Journal of Excellence in Healthcare Management, 3 (1), 1-13.
- Abdulelkarem, A., Hamrouni, A. M., et al. (2009) Risk calculation of developing type 2 diabetes in Libyan adults. Practical Diabetes International, 26 (4), 148-152.
- Benjamin Bradshaw, Paul Evans, Jane Fletcher, Alan T. L. Lee, Paul G. Mwashimba, Daniel Oehlrich, Eric J. Thomas, Robin H. Davies, Benjamin C. P. Allen, Kenneth J. Broadley, Amar Hamrouni and Christine Escargueil.(2008) Synthesis of 5-hydroxy-2,3,4,5-tetrahydro-[1H]-2-benzazepin-4-ones: selective antagonists of muscarinic (M3) receptors. Org. Biomol. Chem., 6, 2138 - 2157
- Amar Hamrouni, N. Gudka, Kenneth Broadley (2006) Investigation of the mechanism for the relaxation of rat duodenum mediated via M1 muscarinic receptors. Autonomic & Autacoid Pharmacology 26(3):275-84
- Amar M. Al-Hamrouni et al. (1997)1‐[(Benzofuran‐2‐yl) phenylmethyl] Imidazoles as Inhibitors of 17α‐Hydroxylase: 17, 20‐lyase (P450 17): Species and Tissue Differences. Pharmaceutical Sciences.
- I have been teaching Pharmacology and Toxicology to Pharmacy students ever since 1987 in Faculty of Pharmacy at Tripoli University in Libya, Ajman University and Al-Ain University in the UAE. I have been also teaching Physiology, Pharmacogenomics, Non-prescription Medications and First aid.
- I have been supervising project students through the years, some of the data of these projects were later published in scientific peer reviewed journals.
- Many of my students have participated in DUPHAT and abudhabi University undergratute competition by posters and oral presentation and they were granted some awads for their valueable work.
- I have been conducting some research through the years and I have some publications in scientific peer reviewed journals. (See under research).
- I was an external examiner for two masters students from faculty of Pharmacy, Ajman University, UAE.
Pharmacology, Toxicology, Physiology, Pharmacogenomics, Non-prescription Medications, First-aid, and Introtoductory Pharmacy Practice experiences (IPPEs) which are Community Pharmacy Training courses.
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all.
This person’s work contributes towards the following SDG(s):
- Member of the International Pharmaceutical Federation (FIP).
- Member of Libyan Pharmaceutical Society (LPS).
- College of Pharmacy Curriculum Committee Chair (2015-2018).
- College of Pharmacy Professional development Committee Chair.
Osteoporosis Related Knowledge and Attitudes: The Scenario in Tripoli, Libya
Published in: Journal of Medical Biomedical and Applied Sciences
Aug 07, 2019
Abstract: Background: Osteoporosis is a worldwide disease, which is characterized by a reduction in bone mass and alteration of its architecture that increases bone fragility and the risk of fracture. Objective: This study aimed to assess knowledge and attitudes towards osteoporosis of undergraduate medical students in Libya. Methods: A cross sectional-based study was conducted at Tripoli University, Libya among medical undergraduate students. The participants’ responses were encoded and descriptive analysis was undertaken, and the chi-squared test was used to ascertain any significant difference among the participants’ responses. Results: More than half (389; 52.1%) of the respondents from three different medical colleges have had some knowledge about osteoporosis. More than three quarters (576; 77.1%) described osteoporosis as brittle bones. The majority, 676 (90.5%) agreed with the statement that women are more likely to get osteoporosis than men. Regarding the knowledge of participants about the link between osteoporosis and other health indicators/conditions, the majority 638 (85.4%) of the respondents did not agree that “osteoporosis is a nonhereditary disease”. Interestingly, 486 (65.1%) of the participants did not agree that osteoporosis affects women only at menopause, and that smoking, alcohol drinking, and pollution were an important risk factors in developing osteoporosis. In reducing the risk of osteoporosis, 725 respondents (97%) selected calcium and 617 (82.6%) selected vitamin D as important nutrients. Conclusions: Medical university students have acceptable but not comprehensive knowledge about osteoporosis related to epidemiology, etiology, pathophysiology and treatment. There is need to continuously revise the medical colleges syllabi and update the courses to reflect recent advances in the management of osteoporosis. Key words: Osteoporosis, Survey, Tripoli University, Menopause
Molecualr modelling studies on alpha-7 nicotinic receptor allosteric modulators yields novel filter-based virtual screening protocol
Published in: Journal of Molecular Graphics and Modelling
Jul 02, 2019
The ɑ7 receptor is a member in the nicotinic acetylcholine receptor (nAChR) which has been implicated in several neurological disorders. Beside normal agonists and antagonists of α7 nAChRs, several studies revealed other types of molecules that are able to activate or deactivate ɑ7 receptors via allosteric binding; those are called positive allosteric modulators (PAMs) or negative allosteric modulators (NAMs), with the former having more pharmacological importance than the latter. Since both types of modulators are believed to bind to the same place in the intracavity of the transmembrane domain, it was important to differentiate between them in terms of structural features and their binding with the target receptor, and then use these specific characteristics as filters to discriminate PAMs from NAMS. To do that, modulators' physicochemical properties were investigated using two databases of known PAMs or NAMs which were then used to elucidate a specific pharmacophore for each class. Interestingly, PAMs were found to be relatively larger and more polar compared to NAMs, which was observed to carry a positive charge with double the number of cases than PAMs. Furthermore. a pharmacophore for each class was developed and the best PAMs pharmacophore was successfully able to pass 94% of tested PAMs and to eliminate 71% of NAMs, while the best NAM pharmacophore was able to pass 82% of NAMs and to filter out 85% of PAMs. Docking these known modulators into the α7 nAChRs allosteric site identified several amino acids that are key for specifically binding PAMs compared to NAMs. Next, these findings were employed in virtual screening and then seeding experiments were conducted to validate the developed pharmacophores usage as filters prior to the final docking. Interestingly, the number of retrieved PAMs in the final docking list was improved by up to five-fold compared to the non-filtered protocol, which clearly indicates for the efficiency of our protocol to pick true PAMs over decoys. Hence, the pharmacophore-based filtering technique developed in this work can act as a valuable tool in the pursuit of new, potent PAM molecules as therapeutically useful modulators of the α7 nicotinic receptors.
The choice of pharmacy profession as a career: UAE experience
Published in: Asian Journal of Pharmaceutical and clinical Research
Apr 14, 2016
The hypoglycemic effect of Libyan Truffle (Terfezia Boudieri) in experimentally induced diabetic rats.
Published in: Tripolitana Medical Journal, Vol 3 No. 1 pp. 1-4.
Apr 28, 2014
Recent studies have shown that many types of mushrooms may have important physiological functions in humans including antioxidant activities, regulation of blood lipid level and blood glucose level. The present study was designed to investigate the antihyperglycemic effect of ethanolic Terfezia boudieri Chatin (TBC) extract on streptozotocin (STZ) induced-diabetic rats. The results showed that, rats which were fed with a concentration of 200mg/kg of TBC ethanolic extract had a significant reduction in the plasma blood glucose (P<0.005). Polydipsia was stronger in the induced diabetic rats which were not fed with TBC extract than in those receiving the T. boudieri extract.
Anti-inflammatory agents of the carbamoylmethyl ester class: synthesis, characterization, and pharmacological evaluation
Published in: Journal of Inflammation Research
Mar 02, 2013
Abstract: In this study, target compounds 5–12 were synthesized via acid amine coupling of ibuprofen and naproxen with methyl ester derivatives of amino acids, namely, l-proline, sarcosine, l-tyrosine, and l-glutamic acid. When tested for anti-inflammatory activity using the acute carrageenan-induced hind paw method in rats, compounds 5–12 showed significantly greater anti-inflammatory activity, in the range of 40.64%–87.82%, compared with a placebo control group (P , 0.001). Among the newly synthesized compounds 5–12, naproxen derivatives 9–12 with anti-inflammatory activity ranging between 66.99% and 87.82% showed significantly higher (P , 0.05) potency than ibuprofen derivatives 5–8 with inhibition in the range of 22.03%–52.91% and control groups of ibuprofen (76.34%) or naproxen (75.59%, P , 0.05). Moreover, derivatives 9–12 derived from naproxen, in particular compounds 9 and 10 which achieved 83.91% and 87.82% inhibition of inflammation, respectively, showed significantly (P , 0.05) higher potency than naproxen derivatives 11 and 12. Notably, among naproxen derivatives 9–12, the gastric ulcerogenicity for 9 (ulcer index 11.73) and 10 (ulcer index 12.30) was found to be significantly lower (P , 0.05) than that of the active ibuprofen and naproxen control groups with ulcer indices of 22.87 and 24.13, respectively. On the other hand, naproxen derivatives 9–11 showed significant inhibition (P , 0.05) of prostaglandin E2 synthesis when compared with the active control group receiving indomethacin, suggesting a correlation between the observed low ulcerogenicity and effect on prostaglandin E2 synthesis for compounds 9 and 10. However, significant inhibition of prostaglandin E2 observed for naproxen derivative 11 (107.51) did not correlate with its observed ulcer index (16.84). Our overall findings for carbamoylmethyl ester derivatives named 5–12 clearly suggest that the compounds showing potent antiinflammatory effect. Keywords: carbamoylmethyl ester, anti-inflammatory, prostaglandin E2, inhibitory propertie
Phytochemical, Antioxidant, Antibacterial and Anti-Inflammatory Investigation of the Methanolic Extract of Amaranthus Tricolor Seed
Published in: Tripolitana Medical Journal, Vol 1, No 2, pp. 94-99
Dec 19, 2012
Prescribing Patterns of Selected Agents at General Medicine Department, Tripoli Central Hospital, Libya
Published in: International Journal of Healthcare Management Vol 3 Issue 1
Mar 01, 2010
Risk calculation of developing type 2 diabetes in Libyan adults
Published in: Practical Diabetes International 29(4):148-151
May 01, 2009
The aim of this study was to identify nationals at risk of developing type 2 diabetes within the next 10 years in some areas across Tripoli Health Authority in Libya. In this questionnaire-based survey, a total of 400 Libyan nationals of both genders were randomly selected from seven areas across the central area of Tripoli Health Authority (Soug El-Juma, Zawet Dahmani, Al-Furnaj, Ain Zara, Al-Madena Centre, Al-Dhahra Centre, and Noflean). All participants approached (400) completed the study and responded to the items of the survey. Based on a modified Finnish Type 2 Diabetes Risk Score test (FINDRISC), 129 (32.3%) were categorised as either at moderate or at high/very high risk of developing diabetes within the next 10 years of life. Among the 129 participants at risk, body mass index was >25kg/m2 in 125 (96.9%) and waist circumferences were high (>88cm for females; >102cm for males) in almost 45% of the women and 22% of the men. We found that in the sample studied the risk of developing diabetes was clear, and there is no doubt that interventions to reduce such risk are a priority rather than a need. Diabetes has a great impact on the health of the nation and also on the future resources of the country in managing the disease and its complications; a health education/health campaign could be one good answer to tackle the problem.
Synthesis of 5-hydroxy-2,3,4,5-tetrahydro-[1H]-2-benzazepin-4-ones: Selective antagonists of muscarinic (M3) receptors
Published in: Organic & Biomolecular Chemistry 6(12):2138-57
Jul 01, 2008
Two approaches to tetrahydro-[1H]-2-benzazepin-4-ones of interest as potentially selective, muscarinic (M(3)) receptor antagonists have been developed. Base promoted addition of 2-(tert-butoxycarbonylamino)methyl-1,3-dithiane with 2-(tert-butyldimethylsiloxymethyl)benzyl chloride gave the corresponding 2,2-dialkylated 1,3-dithiane which was taken through to the dithiane derivative of the parent 2,3,4,5-tetrahydro-[1H]-2-benzazepin-4-one by desilylation, oxidation and cyclisation via a reductive amination. After conversion into the N-tert-butyloxycarbonyl, N-toluene p-sulfonyl and N-benzyl derivatives , hydrolysis of the dithiane gave the N-protected tetrahydro-[1H]-2-benzazepin-4-ones . However, preliminary attempts to convert these into 5-cycloalkyl-5-hydroxy derivatives were not successful. In the second approach, ring-closing metathesis was used to prepare 2,3-dihydro-[1H]-2-benzazepines which were hydroxylated and oxidized to give the required 5-hydroxy-2,3,4,5-tetrahydro-[1H]-2-benzazepin-4-ones. Following preliminary studies, ring-closing metathesis of the dienyl N-(2-nitrophenyl)sulfonamide gave the dihydrobenzazepine which was converted into the 2-butyl-5-cyclobutyl-5-hydroxytetrahydrobenzazepin-4-one by hydroxylation and N-deprotection followed by N-alkylation via reductive amination, and oxidation. This chemistry was then used to prepare the 2-[(N-arylmethyl)aminoalkyl analogues , , and . N-Acylation followed by amide reduction using the borane-tetrahydrofuran complex was also used to achieve N-alkylation of dihydrobenzazepines and this approach was used to prepare the 5-cyclopentyl-5-hydroxy-2,3,4,5-tetrahydro-[1H]-2-benzazepin-4-one and the 5-cyclobutyl-8-fluoro-5-hydroxy-2,3,4,5-tetrahydro-[1H]-2-benzazepin-4-one . The structures of 2-tert-butyloxycarbonyl-4,4-propylenedithio-2,3,4,5-tetrahydro-[1H]-2-benzazepine and (4RS,5SR)-2-butyl-5-cyclobutyl-4,5-dihydroxy-2,3,4,5-tetrahydro-[1H]-2-benzazepine were confirmed by X-ray diffraction. The racemic 5-cycloalkyl-5-hydroxy-2,3,4,5-tetrahydro-[1H]-2-benzazepin-4-ones were screened for muscarinic receptor antagonism. For M(3) receptors from guinea pig ileum, these compounds had log(10)K(B) values of up to 7.2 with selectivities over M(2) receptors from guinea pig left atria of approximately 40.
Investigation of the mechanism for the relaxation of rat duodenum mediated via M1 muscarinic receptors
Published in: Autonomic & Autacoid Pharmacology 26(3):275-84
Aug 01, 2006
1 Relaxation responses of the rat isolated duodenum to the putative M1 muscarinic receptor agonist, McN-A-343, were examined to determine whether the response was due to the release of known non-adrenergic, non-cholinergic relaxant neurotransmitters and to establish the involvement of M1 muscarinic receptors. 2 The role of ATP was examined with the P2 receptor antagonist, suramin, which at 30 μm antagonized the relaxant responses to α,β-methylene ATP. The same dose, however, failed to inhibit the relaxation by McN-A-343. 3 The role of nitric oxide (NO) was examined with the NO synthase inhibitor, NG-nitro-l-arginine methyl ester (l-NAME; 100 μm), which failed to inhibit the responses to McN-A-343. As NO mediates relaxation of the duodenum via cGMP generation through guanylyl cyclase, whether the relaxation by McN-A-343 was also via cGMP was examined with the guanylyl cyclase inhibitor, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ). The relaxation responses to the NO donor, S-nitroso-N-acetyl penicillamine, were inhibited in the presence of ODQ (3 μm), but not those by McN-A-343. 4 Release of γ-aminobutyric acid (GABA) was examined with the GABAA receptor antagonist, bicuculline (10 μm), which shifted the concentration–response curves for the relaxation of the duodenum by GABA to the right. There was a similar degree of shift in the concentration–response curve for McN-A-343 by bicuculline indicating that release of GABA from enteric neurones of the duodenum could explain the relaxation response to McN-A-343. 5 To test whether the muscarinic receptors mediating the relaxation of the duodenum were of the M1 subtype, the susceptibility to the selective competitive antagonist, pirenzepine and the selective muscarinic toxin from green mamba, MT7, was examined. Pirenzepine (1 μm) shifted the concentration–response for McN-A-343 to the right in a parallel fashion with a dose ratio of 33.3 ± 20.2. This yielded a pA2 value of 7.5, which concords with those for other responses reputed to be mediated via M1 muscarinic receptors. The toxin MT7 was used as an irreversible antagonist and following incubation with the duodenum was washed from the bath. An incubation time of 30 min with 100 nm of MT7 caused a significant parallel shift in the concentration–response to McN-A-343 confirming the involvement of M1 muscarinic receptors. 6 This study has confirmed that McN-A-343 relaxes the rat duodenum via muscarinic receptors of the M1 subtype and that these receptors are probably located on enteric neurones from which their stimulation releases GABA.